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Bismuth is an essential metalloid used for centuries in human life, as an ingredient of paper, cosmetics, and medicines and of some industrial and agricultural agents. Its toxicity is low, as it is incorporated into various structures of living organisms by means of enzymes and other metallothioneins. The distribution of biomethylation in cell protein may indicate the cellular distribution of bismuth. Here, we have investigated the bismuth binding to the thiol groups of metallothioneins from rat liver and striatum by using LC-ESI-FTMS analysis. The thiol groups are sequentially accessible to bismuth. The dissociation constants of the thiol groups in the rat liver and striatum metallothionein are 0.29+/-0.03 and 0.04+/-0.001, respectively. We also observed the significant differences between the positive and negative ionization modes for thiol binding. We have developed a quantitative PCR method to measure the level of metallothionein expression using its Rsa I gene. The expression of the thiol-containing metallothionein gene is increased in the liver and decreased in the striatum of rats with the treatment of bismuth, indicating that the thiol groups of metallothionein might be essential in the biomethylation of bismuth in the living bodies.Braybrook City Railways 940 class
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