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To investigate the methylation status of retinoblastoma (Rb) gene in lung carcinomas, a total of 50 primary lung carcinoma cases were collected, including 15 cases of adenocarcinomas, 16 cases of squamous cell carcinomas, 12 cases of small cell lung carcinomas, and 7 cases of large cell lung carcinomas. The methylation status of the 5′ CpG island of Rb gene was determined by methylation-sensitive restriction enzyme Hpa II assay and Southern blotting. Using different oligonucleotide primers, four methylation-specific PCR products were then amplified and detected by two-dimensional gel electrophoresis. A total of 24.0% cases (12/50) showed methylation of Rb gene. There was no significant association between the methylation status and age, sex, smoking history, and histological type. But more cases were observed in female (66.7%) than in males (33.3%). It was suggested that the inactivation of Rb gene by methylation may be involved in the development of lung carcinoma in females.//
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Aortitis syndrome (AAS) is a heterogeneous group of inflammatory diseases of the aorta with different clinical manifestations, including acute aortic syndromes, upper extremity pain, and aortic dilatation. Although endocardial fibroelastosis (EFE) is known to be one cause of aortic valve dysfunction in patients with acute aortic syndromes, the clinical relevance of diffuse wall thickening and aortic dilation is still unknown. We hypothesize that the apparent diffusion coefficient (ADC) values of aortic wall and intimal/extramural thrombi are significantly lower than those of adjacent normal aortic wall in AAS. We evaluated six patients (two acute aortic syndromes, three acute periaortic phlegmon, and one abdominal aortic aneurysm) and five healthy controls. All patients underwent cardiac magnetic resonance imaging (MRI) including diffusion-weighted imaging (DWI). Three were additionally scanned using enhanced MRI, and two underwent postcontrast administration. The ADC values were compared with normal aortic wall. The mean ADC value of acute periaortic phlegmon (0.74 +/- 0.02 x 10(-3) mm(2)/s) was significantly lower than that of normal aortic wall (1.05
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