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During the reign of Akbar, Nawab Jai Singh of Jaipur could not tolerate the rise of the Marathas from the south..
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To study the direct effect of cAMP on apical Cl- channels in renal cortical collecting duct (CCD) cells, patch clamp technique was used to record Cl- currents under whole cell configuration. The pipette solution contained 10 microM 9-anthracene-b-methyl-carbamil (Cab). HCO3(-)-free solution (HEPES-buffered HCO3(-)-free solution was used for recording HCO3- -dependent currents. When the bath solution contained 10 microM cAMP, cab-sensitive Cl- currents were also observed in a non voltage-clamped cell. In cAMP-free solution, cab-sensitive Cl- currents were not observed, and voltage-dependent Cl- currents were recorded. The cab-sensitive Cl- current was inhibited by hemicholinium-3 and elevated by 8BrcAMP. These results suggest that the cab-sensitive Cl- currents in CCD cells are most likely due to Cl- channels that are coupled to a Cl-/HCO3- exchanger.Q:
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