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Alkylating agents are a diverse group of chemicals that react with DNA to produce crosslinks, DNA-DNA or DNA-protein crosslinks or adducts. Chemically the alkylating agents can be classified as monofunctional alkylators, which, by definition, cause DNA damage but they do not form covalent bonds with the DNA backbone. The trifunctional alkylators, on the other hand, can form interstrand or intrastrand crosslinks with DNA. Trialkylating agents are often used to synthesize highly reactive intermediates for use in chemical reactions, such as the cross-linking of proteins to DNA. The alkylating agents can also be classified as charged or noncharged according to their ionic properties. Charged alkylating agents in general act by alkylating DNA directly or indirectly via an intermediate reactive species. The noncharged alkylating agents generally covalently bind to nucleophilic DNA sites. In vivo, DNA is protected by the cell’s enzymes and macromolecules so that only the DNA that is exposed to the cellular environment is free to alkylate or crosslink. The DNA can be separated into classes which differ according to their chemical natures, covalent binding properties, DNA reactivity and cellular susceptibility. This review article will discuss the in vitro and in vivo genotoxicity testing methods to identify the alkio-cation, alkylating agents that may be carcinogens.Your girl’s going to be back in my life
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