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Bioinformatics.r.Large-scale isolation and purification of antibodies are known in the art. Representative exemplary processes are discussed in U.S. Pat. No. 5,656,039 to Porgador, et al., U.S. Pat. No. 5,837,242 to Takamura, et al., U.S. Pat. No. 5,869,619 to Barry, et al. and U.S. Pat. No. 5,869,619 to Barry, et al.
For the isolation and purification of an antibody, large amounts of cells and culture media are typically required. For example, to prepare a therapeutic dose of an antibody (about 1-2 grams) from a cell culture, about 4 liters of medium is typically required. To obtain this number of liters, a large cell culture flask is typically used, for example, a 125 ml or a 250 ml flask, and for small scale applications, from about 1 to about 4 liters of medium may be required. As one example, for a 250 ml or 125 ml cell culture flask, 1 to 2 liters of medium is typically required. For larger cell culture flasks, as one example, from about 4 to about 10 liters of medium may be required. When using a cell culture vessel or container, two types of waste, cell and media, can be generated. For cell culture, cell debris can be released into the medium, with the cell debris generally defined as being at least 10-50 xcexcm in size. Cell debris includes damaged cells, cell death bodies, and insoluble cell components. Cell debris generally does not occur in concentrations high enough to affect the cell culture. In contrast, large amounts of
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